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Associate Scientist - South San Francisco, United States - Collabera
Description
Job Description
Job DescriptionJob Title: Associate Scientist
Location: South San Francisco (Onsite)
Duration: 12+ months
Top 3 Must Have Skill Sets:
1. BS or MS in science-related field + 2 years laboratory experience with significant hands-on laboratory work demonstrating mastery of micro-pipetting (single and multi-channel), sample manipulation in small tubes (microcentrifuge) and multi-well plates, SDS-PAGE; proficient using other basic lab equipment (e.g., pH meter, balances, centrifuges); making basic laboratory calculations, (e.g., for making standard buffers from dry chemical ingredients or liquid stock solutions), strong fundamental training in sterile technique and knowledge of safeguards for handling mammalian cultures.
2. Ability to follow established laboratory protocols and adhere to data standards; attention to detail and careful execution of laboratory tasks are essential to our team
3. Preferred skills include familiarity with automated chromatography hardware/software (e.g. AKTA) and previous experience culturing mammalian cells.
4. Strong written and oral communication skills
Day to Day Responsibilities:
1. Membrane Protein Purification
a. Cell lysis and membrane pellet preparation
b. Perform batch affinity chromatography using anti-FLAG, Strep-Tactin, and Ni-NTA His affinity resin
c. Use of AKTA chromatography instrumentation for protein purification
d. Running and imaging SDS-PAGE gels and western blots
e. Use of spectrophotometry and colorimetric assays for concentration determination
f. Use HPLC for protein quality control
Cell Culture
RNA
DNA
PCR Performing
2. Protein expression
a. Culturing mammalian cells such as HEK293 and/or Expi293
b. Measuring cell viability using Vi-cell
c. Running and imaging SDS-PAGE gels and western blots
d. Keeping records of data and procedures
3. Inventory Management and general routine laboratory activities
a. Register protein lots into/out of internal storage system, as needed
b. Check stocks of required consumables and order and stock when necessary (i.e. common reagents)
c. Organize and set up -80C storage DNA inventories
Pay rate: $33- $35per hour
Company DescriptionCollabera is a Global Digital Solutions Company providing Software Engineering Solutions for the world's most tech-forward organizations in the areas of Engineering, Cloud and Data/AI. With its roots serving the engineering needs of the world's most recognized businesses in Technology, Financial Services, Telecom and Healthcare, Collabera today operates across 60 locations in 11 countries, serves 30% of the Fortune 500, and has exceeded the industry growth rate by 3-4x for several years.
