Research Assistant I - La Jolla, United States - Salk Institute

Description

Job Summary

The Research Assistant I is responsible for research support and some lab management duties in support of the Principal Investigator's (PI) studies along with duties involving molecular biology, biochemistry, protein production, purification. Duties include performing basic molecular biology and biochemical experiments for protein production, purification, and for biochemical assays in support of research projects, assisting the postdoctoral associates with experimental design and direction, supervising and assisting with training of junior lab staff (junior technicians, students and volunteers), as necessary. Incumbent is also responsible for assisting with general laboratory duties, equipment upkeep and repair, ensuring compliance with all Salk policies, and working with collaborators to achieve specific research goals.

Works on problems in which analysis of situations or data requires an in-depth evaluation of various factors. Exercises judgement within broadly defined practices and policies in selecting methods, techniques, and evaluation criteria for obtaining results. May determine methods and procedures on new assignments. May provide supervision, training and guidance to lower-level personnel. Work is done under periodic supervision.

Who We Are

The Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community comprises a diverse and talented group of individuals, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.

What Your Key Responsibilities Will Be

• General protein production and purification for several projects.
• Works with the E. coli systems for protein production. Utilize different expression protocols to produce proteins.
• Performs various techniques in molecular biology such as: DNA extraction and analysis, western blotting, PCR, gel electrophoresis, cloning, etc.
• Uses a variety of equipment for protein purification, including the Akta FPLC, a gradient station, and preparatory cell.
• Employs different purification protocols, including for size exclusion chromatography, ion exchange chromatography, affinity purification, gradients, and others as per research demands.
• Performs biochemical binding assays, activity assays, evaluating formation of protein complexes.
• Presents data at sub-group and at group meetings, and presents research papers in journal clubs.
• Writes laboratory protocols for experiments and ensures their compliance within the laboratory.
• Assists with stocking, ordering, maintaining, and keeping track of resources, chemicals, and supplies; assists with maintaining inventory.
• Assists with performing regular maintenance and calibration of equipment; ensuring equipment is operational; assists with instrument repairs when necessary.
• Assists with cleanups.
• Trains incoming or lower-level staff, as appropriate
• Communicates with vendors and Salk personnel, operational and procedural.
• Trains staff on specific molecular biology and related techniques. Trains laboratory personnel in proper use of laboratory equipment and equipment maintenance and assists in hiring process of new staff.
• Maintains logbooks for major equipment (e.g. FPLC).
• Performs other duties as assigned by management.

What we Require

• No professional experience required.
• Education: Bachelor's degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelor's degree in a different discipline.
• Computer skills required: (Select all applicable): Accounting Software; Contract Management Systems; Development Software; Human Resource Systems; Inventory Software; Microsoft Office; Payroll Systems; Project Management Software)
• Other skills (preferred):
  • Prior experience or internships in a laboratory setting.
  • Prior experience with histology, which may be gained from coursework and/or work experience.
  • Basic knowledge of laboratory procedures and safety practices.
  • Familiarity with specific laboratory techniques or equipment relevant to the Institute's operations.
  • Knowledge of relevant regulations and compliance standards.
  • Specific interests and advanced skills in biochemical methods.

What We Can Offer

The expected pay range for this position is $18.03-$21.00 per hour. Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.

Benefits

Salk Institute offers competitive benefits, including medical, dental, vision, retirement, paid time off, tuition reimbursement, patient advocacy services, and transit/parking program.

Salk Values

The Salk Community, both scientists and administrators, worked together to define values that we believe support Salk's pursuit of excellence. To be truly the best scientific institution requires not only incredible discoveries, but a common understanding of how we should work together to enable those discoveries.

The acronym "I CARE" provides a simple way to remember each of the values and reminds each of us of the importance of what we do each day.

Equal Employment Opportunity Statement

The Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students, employees, applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace.

Accordingly, the Institute prohibits harassment and discrimination in employment on the basis of, and considers all qualified applicants for employment without regard to, actual or perceived race, color, religion, religious creed (including religious dress and grooming practices), national origin, ancestry, citizenship, physical or mental disability, medical condition (including cancer and genetic characteristics), genetic information, marital status, age, sex (including pregnancy, childbirth, breastfeeding, or related medical conditions), gender, gender identity, gender expression, sexual orientation, veteran and/or military status (disabled veteran, veteran of the Vietnam era, other covered veteran status), political affiliation, and any other status protected by state or federal law.