Research Assistant I - La Jolla, United States - Salk Institute for Biological Studies

Mark Lane

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Mark Lane

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Description

SUMMARY:


The Research Assistant I position is responsible for, with limited instruction for routine work, performing various cell and molecular biology and data handling techniques; library prep, mouse handling, minor mouse surgeries, mouse breeding and weaning, genotyping, flow cytometry and cell sorting, ELISA, RNA expression analysis in support of the Principal Investigator (PI) and lab research goals.

Range of duties include, but is not limited to, data analysis and data base management, maintenance of tissue, DNA, and other experimental samples, assisting with general laboratory maintenance.

Duties include performing research and analysis on projects that involve the study of human and murine B cells. Carries out and analyzes experimental results with instruction from the PI.


DUTIES AND RESPONSIBILITIES:


  • Library prep for various omics platforms.
  • Plasmid assembly and protein purification, and cloning.
  • Handling of tumor specimens in human and murine models.
  • Perform various techniques in molecular biology such as: RNA extraction and analysis.
  • Maintenance of data sets, lab supplies, and general lab operations.
  • Perform tumor studies in mice which include, but are not limited to, xenograft of tumor cell lines and injection of oncogenic lentivirus
  • Maintenance of mouse colony including monitoring and measuring of tumors
  • Assist with cell maintenance.
  • Build figures
  • Clean glassware and equipment as needed
  • Prepare standard media and solutions
  • Assist in weekly inventory, supply stocking, and cleaning.
  • Assist in Hazardous, Biohazardous, and Radioisotope waste disposal and maintain safety records
  • Performs other related duties as assigned

SUPERVISORY RESPONSIBILITIES:


  • This job has no supervisory responsibilities.

QUALIFICATIONS:


  • Minimum of 1 years of experience in a lab either as technician or assistant.
  • Computer skills required: Inventory Software; Microsoft Office; Project Management Software

SALK VALUES:

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Integrity
  • Guides our moral compass and underpins our every action. We do what is right in all situations for no other reason than because it is right. We uphold honesty and ethical behavior and make good on our commitments. We understand that words and deeds matter and that integrity leads to trust.
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Community:


  • Enables us to harness the power of our collective talents. We work together towards common goals. We recognize the importance of diversity where everyone's ideas, identities, backgrounds, and perspectives are valued and pave the paths that propel us forward. We understand our pioneering role in the broader scientific community and take pride in our efforts to have a positive impact on the world.

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Accountability

  • Keeps us focused, and keeps us honest. We are responsible not only for our own success, but the success of our teams and the entire Institute. We consider how the actions we take and the decisions we make in our own work may impact others.

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Respect

  • Creates the foundation of trust and brings out the best in us all. We value the experiences, identities, and feelings of those we work with, regardless of their position or their relationship to us. We provide feedback in a constructive manner, use appropriate language, and allow others to share their thoughts and ideas without fear of ridicule.

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Empowerment

  • Amplifies all voices and inspires the pursuit of greatness. We lift each other up, enabling us to grow continuously and live to our fullest potential. We support the expression of ideas, encourage selfadvocacy, inspire confidence and recognize that an environment of mutual respect is critical to our collective success.

COMPETENCIES:

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Adaptability
  • Adapts to changes in the work environment; Manages competing demands; Changes approach or method to best fit the situation; Able to deal with frequent change, delays, or unexpected events.

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Analytical

  • Synthesizes complex or diverse information; Collects and researches data; Uses intuition and experience to complement data; Designs work flows and procedures.

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Attendance/Punctuality

  • Is consistently at work and on time; Ensures work responsibilities are covered when absent; Arrives at meetings and appointments on time.

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Customer Service

  • Manages difficult or emotional customer situations; Responds promptly to customer needs; Solicits customer feedback to improve service; Responds to requests for service and assistance; Meets commitments.

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Dependability

  • Follows instructions, responds to management direction; Takes responsibility for own actions; Keeps commitments; Commits to long hours of work when necessary to reach goals; Completes tasks on time or notifies appropriate person with an alternate plan.

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Initiative

  • Volunteers readily; Undertakes selfdevelopment activities; Seeks increased responsibilities; Takes independent actions and calculated risks; Looks for and takes advantage of opportunities; Asks for and offers

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