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    Sr. Scientist I - North Chicago, United States - AbbVie

    AbbVie
    AbbVie North Chicago, United States

    1 week ago

    Default job background
    Full time
    Description
    Job Description

    Key Responsibilities

    • Design, execute, & interpret critical experiments to answer scientific questions.
    • Generate & test hypothesis by designing, performing, & interpreting laboratory-based experiments.
    • Read literature critically & analyze genomic data to support these activities.
    • Develop in vitro & in vivo screening assays in collaboration with therapeutic area scientists by generating cell lines, developing lentiviral & RNP delivery methods, & integrating & optimizing screening protocols.
    • Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types.
    • Perform pooled & arrayed CRISPR screens with FACS-based molecular & functional assays on immortalized cell lines, primary cells, & iPSC-derived cells.
    • Work closely with NGS & bioinformatics scientists to process genomic samples, analyze screening results, & generate screening hits for follow-up studies.
    • Investigate novel screening hits in the context of disease biology by generating & testing hypothesis using a variety of biochemical, molecular, cellular, & genomic assays.
    • Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot.
    • Map candidate genes to known disease genetic networks through experimental & bioinformatic approaches.
    • Learn, understand, & master new experimental techniques, & act as a resource or mentor for others. Perform RNA-sequencing, RNAi (shRNA/siRNA), & CRISPR gene editing.
    • Collaborate and communicate with cross-functional scientists on experimental designs and results and scientific goals.
    • Report research progress to management on a regular basis, provide project updates to cross-functional project teams, and provide scientific presentations to department and other internal scientific forums.
    • Seek and advance opportunities to present research results at external scientific conferences & publish high-impact articles in peer-reviewed journals.
    Qualifications

    Must possess a PhD or foreign academic equivalent in Biochemistry and Molecular Biology, Biological Sciences, Chemistry, or a closely related scientific field

    with an academic or industrial background in:

    1. transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types;
    2. molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot; &
    3. RNA-sequencing, RNAi (shRNA/siRNA), & CRISPR gene editing.

    Apply online at Refer to Req ID: REF23197A.

    Additional Information

    We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees. This job is eligible to participate in our short-term and long-term incentive programs.

    AbbVie is committed to operating with integrity, driving innovation, transforming lives, serving our community and embracing diversity and inclusion. It is AbbVie's policy to employ qualified persons of the greatest ability without discrimination against any employee or applicant for employment because of race, color, religion, national origin, age, sex (including pregnancy), physical or mental disability, medical condition, genetic information, gender identity or expression, sexual orientation, marital status, status as a protected veteran, or any other legally protected group status.

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