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    Postdoctoral Scholar - Portland, United States - OHSU

    OHSU
    OHSU Portland, United States

    1 week ago

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    Description
    Department Overview

    The Barad lab seeks a postdoctoral scholar to investigate cytoskeletal reorganization during intracellular bacterial infection using cellular cryo-electron tomography.

    The scholar will receive training in sample preparation, tilt series collection, and data analysis for cryo-electron tomography, as well as development of computational tools for the quantitative analysis of membrane and filament organization.

    In addition to cryo-electron tomography, the project will incorporate correlative cryo-fluorescence microscopy as well as live-cell fluorescence microscopy.

    Applicants should send a cover letter describing research experience and interests, a CV, and contacts for references to Benjamin Barad ).

    The Barad lab explores mechanisms of mammalian membrane remodeling and organellar reorganization during bacterial infection.

    The primary technique used in the lab is cellular cryo-electron tomography, and the lab is invested in methods development advancing tomography as a scale-spanning technique for contextual structural and cell biology.

    Projects in the lab may also involve classical structural biology experiments (primarily single-particle cryo-EM and x-ray crystallography), light microscopy, volume EM, and biophysical characterization.

    Most projects in the lab involve significant computational analysis. Preference will be given to applicants with expertise in either cryo-electron microscopy or tomography or computational image analysis.

    Function/Duties of Position


    • Design and conduct independent research projects related to cytoskeletal reorganization during bacterial infection.
    • Assist in training and supervision of new lab personnel.
    • Maintain an accurate lab notebook detailing all experimental and computational work completed.
    • Maintain compliance documentation for tissue culture and infection work.
    • Attend Lab meeting, make contributions to grants and publications, present research at conferences.
    • Other responsibilities as assigned by the supervisor in furtherance of the lab's research and the university's missions and goals of teaching, research, patient care, outreach and public service.
    Required Qualifications


    • PhD in related field
    • Research experience in biochemistry, structural biology, biophysics, or cell biology
    • Working knowledge of scientific software workflows
    • Demonstrated independence preparing and delivering oral presentations and manuscripts
    Preferred Qualifications


    • Experience with research in CryoEM or CryoET, bacterial infection experiments, and/or bioimage analysis.
    • Cell culture techniques
    • Light or electron microscope operation
    • Filament analysis
    • Programming with python or matlab
    • Experience with machine learning workflows
    Additional Details

    Individuals are expected to work on-site to conduct sample preparation and light and electron microscopy experiments.

    Additional safety training will be needed for work with cell lines and bacterial infection experiments in a BSL-2 environment, and for cryogenic sample preparation and handling.


    Personnel will need to work at lab benches, desks, computers and/or microscopes for up to 4 hours at a time.

    Ability to perform repetitive lab manipulations (gripping, grasping, reaching, pulling), move lab equipment under 25 lbs with accommodation, perform image analysis operation and help others with equipment use.

    Personal protective equipment must be worn as required. (Gloves, mask/respirator, eyewear, lab coat, etc).

    All are welcome

    Oregon Health & Science University values a diverse and culturally competent workforce.

    We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status.

    Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at or
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