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    Research Technician-Lab Manager - New York, United States - Icahn School of Medicine at Mount Sinai

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    Healthcare
    Description

    Job Summary

    Lab of Dr. Prashant Rajbhandari in the Diabetes, Obesity, and Metabolism Institute (DOMI) has an opening for a research lab technician-manger position. Rajbhandari Lab is broadly interested in 1) Immune cell regulation of lipid metabolism (Cell 2018), 2) developmental process of brown/beige and white fat (JCI 2017), 3) mammary gland biology and metabolism (Nature 2023), and 4) crosstalk between adipose and other metabolic tissues (esp liver) in the setting of obesity (Nature Medicine, Cell Metabolism 2018,2019). To address these topics, the lab uses cell and molecular biology, biochemistry, next-gen sequencing (ChIP-, RNA-, ATAC-seq), animal model, single cell/nuclei RNA sequencing, and imaging. The broader significance of the work in Rajbhandari Lab is that obesity is linked to various diseases and by studying how cellular and molecular pathways are perturbed in the setting of obesity might provide better understanding of this disease.

    Duties and Responsibilities

    1. Standard Molecular Biology techniques (PCR, recombinant gene cloning, site directed, mutagenesis, Gibson assemble cloning (preferred))

    2. Preparation of reagents and solutions and buffers for experiments.

    3. RNA and protein extraction for qPCR and western blot, respectively.

    4. Mammalian cell culture techniques.

    5. Animal handing, colony management, and genotyping (required).

    6. Eager to learn, operate, and manage lab equipment.

    7. Lab ordering duties

    8. Lab managerial skills

    Minimum Education

    Bachelor's or Master's Degree in life sciences, molecular biology, biochemistry, premed, or related field

    Minimum Related Experience

    (e.g. 1 year in research environment)

    2 years in a research environment or 1 year plus Master's Degree in sciences or related field

    Minimum Licensing Requirements

    (provide name and agency)

    None

    Minimum Certification Requirements

    (provide name and agency)

    None

    Minimum Computer Skills

    Microsoft Word

    Microsoft Excel

    Microsoft PowerPoint

    Google Docs

    Graphpad Prism

    General Skills

    Excellent organizational, problem solving and analytical skills

    Excellent verbal, written and interpersonal communication skills

    Knowledge of standard research concepts



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