- PhD or equivalent
- Demonstrated experience working in a basic or translational research laboratory
- Willingness to work with pig models of brain injury.
- Demonstrated experience working in and fostering a diverse faculty, staff, and student environment or commitment to do so as a staff member at VCU.
- Demonstrated experience working in a translational or basic research lab work a focus on traumatic brain or spinal cord injury.
- Demonstrated experience working with microglial assessments in histological samples and brain tissue.
- Demonstrated experience with live animal surgery.
- Demonstrated experience training students in wet lab research.
- Demonstrated bioinformatics experience, specifically in regard to single-cell RNA sequencing data.
- Demonstrated ability to help guide a project independently.
- Demonstrated experience drafting, editing, and publishing manuscripts.
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Postdoctoral Fellow - Richmond, United States - State of Virginia
Description
Position Summary:
A postdoctoral position is available in the Lafrenaye lab to work with a translational pig model of traumatic brain injury.
Investigations will focus on social and sensory changes following diffuse brain injury and how these changes are associated with microglial-axonal interaction alterations.
This postdoc will work with a collaborative research group (both in the lab, at VCU, and at external institutions) to help guide these studies, perform animal surgeries, histological and molecular processing/assessment, and single-cell RNA sequencing for experiments of traumatic brain injury pathology in a higher order translational model.
The ideal candidate will have experience with animal surgery, microglia assessments (molecular and/or histological), RNA sequencing/bioinformatics, and/or assessments of diffuse brain-injury-induced pathologies.
The duration of the postdoc is dependent upon funding.Core Responsibilities:
Responsibility 1 (_30_%): Assist with and conduct pig surgical and behavioral procedures.
o Task 1:
Conduct the surgical preparation, traumatic brain injury induction, and physiological monitoring of pigs.
o Task 2:
Conduct behavioral assessments in pigs.
o Task 3:
Participate in the post-injury recovery monitoring and assessment of pigs.
Responsibility 2 (_50%): Quantitative histological, molecular, behavioral, and genetic analysis of pig and human post-mortem tissue
o Task 1:
Prepare, process, and analyze pig and human tissue microscopic samples.
o Task 2:
Prepare, process, and analyze pig brain for molecular assessments, including western blotting and ELISAs.
o Task 3:
Work with the PI and various collaborators to prepare, process, and analyze RNA sequencing data from pigs and humans.
o Task 4:
Participate in analyzing and train undergraduates to analyze the recorded behavioral assessments.
Responsibility 3 (_5%): Manuscript and grant preparation
o Task 1:
Assist in manuscript and grant preparation, writing, and editing.
Responsibility 4 ( 10_%): Assisting in training students in the laboratory
o Task 1:
Mentoring trainees with technical issues involved animal handling, surgery, behavioral tasks, and other quantitative assessments used in the lab.
o Task 2:
Properly demonstrating safe lab techniques and best practices.
o Task 3:
Engaging with trainees about proper research conduct, grant and/or manuscript writing, and other "soft" skills necessary for successful research.
Responsibility 5 (5_%): Assist with laboratory maintenance and management.o Task 1:
Assist the PI and lab manager with day-to-day maintenance and workflow of the lab
o Task 2:
Assist the PI and lab manager with verifying that the laboratory and surgical spaces remain clean and functional.
o Task 3:
Help the PI update and implement best practices for data and protocol management.
Required Qualifications:
Preferred Qualifications:
The School of Medicine continuously strives for our workplace and learning environment to reflect the demographic and social milieu of the communities we serve.
This is a restricted position with no set end date and continued employment is dependent upon project need, availability of funding, and performance.
