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    Systems Validation Scientist 3 - San Jose, United States - MillenniumSoft

    MillenniumSoft
    MillenniumSoft San Jose, United States

    6 days ago

    Default job background
    Full time
    Description

    Roles & Responsibilities

    Must haves on the resume:
    1. Best practice experience doing antibody methods for antigen labeling (, flow cytometry, western blots, ELISA, ELISPOT)
    2. Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
    3. Ability to purify leukocytes from mammalian-derived samples (, venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
    4. Ability to stain cells in tubes and plates for intracellular and extracellular antigens.

    Responsibilities:
    The Systems Validation Scientist II will play a critical role in preparing samples for flow cytometric studies.

    Responsible for:

    • Maintaining the health, expansion, and inventory of cell lines

    • Performing cryopreservation and thawing of mammalian cells

    • Purification of leukocytes from mammalian-derived samples (, venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.

    • Staining of cells in tubes and plates for intracellular and extracellular antigens.

    • Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents.

    • Counting cells via hemocytometer and/or proprietary cell counters.

    • Preparing buffers, media, and other reagents.

    • Maintaining inventory of critical laboratory supplies (consumables and reagents) in the lab

    • Attend meetings, as needed.

    Experience Required


    • At least 1-2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines.

    • Best practice experience doing antibody methods for antigen labeling (, flow cytometry, western blots, ELISA, ELISPOT)

    • Experience working with primary cells derived from various mammalian species.

    Skills & Certifications


    • Proficient with phase-contrast and brightfield microscopy.

    • Excellent aseptic technique.

    • Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies.

    • Ability to calculate basic laboratory calculations.

    • Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency.

    • Ability to read and understand Standard Operating Procedures

    • Ability to interact with researchers for delivery of cells.

    • Ability to maintain a clean work area.

    • Knowledge of GLP/GDP guidelines.

    • Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)

    • Excellent written and oral communication skills.

    Eligibilities & qualifications

    Qualifications:
    The successful candidate will possess:

    • A minimum of a bachelor's degree in Microbiology, Biology, Biochemistry, Immunology, or a related field.

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