Research Scientist Ii - Milwaukee, United States - Medical College of Wisconsin

Medical College of Wisconsin

Verified Company

Milwaukee, United States

3 weeks ago

Posted by:

Mark Lane

beBee recruiter

Description

Research Scientist II

Requisition ID #:

Job Category:
Research- Employment Type: Full Time- Experience Level: Experienced (non-manager)- City, State: Milwaukee, WI- Work Location: Medical Education Building- Department: Cell Biology, Neurobiology and Anatomy- Education: Doctorate DegreePosition Description:

Every day, in ways both big and small, the Medical College of Wisconsin (MCW) is impacting lives for the better.

MCWchangemaking for a healthier Wisconsin. In the role of
a** Research Scientist II
you will be
working in Cell Biology, Neurobiology and Anatomy.

Purpose

Primary Functions

Plan and perform common and advanced molecular biology techniques: qPCR, cloning, subcloning, chromatin immunoprecipitation, luciferase assay, RNAi, development of knockout cell lines and mouse models, FACS
Plan and perform cell biology techniques, including protein purification techniques (e.g. HPLC), bacterial and mammalian cell expression, Western blotting, coimmunoprecipitation, and multiplexed ELISA techniques.
Plan and perform cell biology techniques, including iPSC culture and differentiation into various more mature phenotypes (specifically cardiomyocytes), plasmid transfection and viral infection into cells, isolation of adult cardiomyocytes, and immunohistochemistry.
Design, oversee, and execute preclinical trials of new therapies in cell and animal models of disease, including animal husbandry, in vivo luminescence imaging, and behavioral experiments. Animal disease models include mouse models (Duchenne muscular dystrophy, heart disease) and hamsters (obesity and COVID19), the latter of which will require BSL3 experience.
Plan and perform statistical analyses of next generation sequencing techniques, including RNA-Seq, CHIP-Seq, ATAC-Seq, and Next Gen Sequencing Analysis
Prepare scientific reports, outcome findings and scientific manuscripts. Assist in outcome interpretation and composition of the results sections of manuscripts and reports.
Participate in meetings with principal investigator and research staff and evaluate/interpret the validity of data, develop methodologies, evaluate lab procedures.
Train students, residents and postdoctoral fellows in specific research protocols; develop training materials.
Develop, implement and maintain a computerized record system for reporting results.

Knowledge - Skills - Abilities
Applicants should have extensive experience with common lab techniques such as qPCR, Western blotting, and cell culture. Experience with animal handling, transcriptomics, proteomics, and/or epigenetics are strongly urged to apply.

Applicants must be highly self-motivated and possess the ability to think and work independently. Strong scientific communication skills, open to learning new science, and scientific integrity are required.

Preferred Schedule:
Full Time

Position Requirements:

Minimum Required Education:
PhD or Master's with experience

Minimum Required Experience: 5 years

Field:
Transcriptomics or Epigenetics experience

**Why MCW?

Outstanding Healthcare Coverage, including but not limited to Health, Vision, and Dental. Along with Flexible Spending options
403B Retirement Package
Competitive Vacation and Paid Holidays offered
Tuition Reimbursement
Paid Parental Leave
Pet Insurance
On campus Fitness Facility, offering onsite classes.
Additional discounted rates on items such as: Select cell phone plans, local fitness facilities, Milwaukee recreation and entertainment etc.

MCW as an Equal Opportunity Employer and Commitment to Non-Discrimination