- Gene Editing:
- Using CRISPR/Cas9 for genome engineering of cell lines, generation of CRISPR/Cas9 gene targeting reagents, selection of edited cell lines by single cell cloning and flow cytometry, PCR and DNA sequencing for validation of edited cell lines.
- Molecular Biology:
- Molecular cloning, PCR, qRT-PCR, plasmid extraction and DNA sequencing.
- Assist in the generation of next-generation sequencing libraries for ChIP-seq, ATAC-seq, Cut&Run and HiChIP experiments.
- Tissue Culture:
- Maintenance of cell lines and preparation of tissue culture reagents.
- Seeding of experimental plates for plasmid transfection and lentivirus & AAV transduction.
- Harvest cell lysates for DNA, RNA and protein analysis.
- Mouse Colony Management:
- Maintenance and breeding of transgenic mice that include weaning, sexing and breeding of mice, genotyping and record keeping of mice produced for the lab.
- Prepare harvested organs and tissues for biochemical and histological analysis.
- Assist with surgical procedures for embryonic kidney organ isolation and ex vivo culture.
- Responsible for drug preparation and administration, when necessary.
- Data Analysis:
- Prepare written and graphical descriptions of results to discuss with PIs.
- Review scientific literature and technical documents.
- Collaborate with faculty and postdocs in the lab regarding problem solving and data analysis.
- Present routine summary reports on research protocols to faculty and postdocs.
- Troubleshoot methods and techniques for potential improved outcomes.
- Use R for statistical analysis and graph plotting.
- Other duties as assigned.
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Research Support Specialist - Stony Brook, United States - Stony Brook Medicine
Description
Required Qualifications (as evidenced by an attached resume):Bachelor's degree (foreign equivalent or higher) in biology or a related field of science, in hand by May 2024. Experience with research activities which may include cell biology, genetics, biochemistry, and/or molecular biology (student experience may be considered).Preferred Qualifications:Experience in molecular biology techniques which may include PCR, cell culture, mouse husbandry, sectioning for IF, IHC and ISH, and/or Western blotting. Experience with CRISPR/Cas9, gene editing and/or next-generation sequencing. Experience with Excel, Photoshop and/or ImageJ.Brief Description of Duties: We are seeking a highly self-motivated individual with a positive attitude for a Researcher Support Specialist position to join our group studying Polycystic Kidney Disease (PKD) in the Division of Nephrology and Hypertension, Department of Medicine. This is a laboratory-based position focused on the study of transcriptional regulation and signaling mechanisms in PKD. The successful incumbent will work closely with a research faculty and assist experiments in research projects.The successful incumbent will have excellent interpersonal skills, exercising strong organizational and time-management skills with an exceptional attention to detail. Essential for this role is the adeptness to work independently as well as part of a team with a collaborative approach to problem solving.
Duties:
For this position, we are unable to sponsor candidates for work visas.
Resume/CV and cover letter should be included with the online application.
Stony Brook University is committed to excellence in diversity and the creation of an inclusive learning, and working environment. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion, sex, pregnancy, familial status, sexual orientation, gender identity or expression, age, disability, genetic information, veteran status and all other protected classes under federal or state laws.If you need a disability-related accommodation, please call the university Office of Equity and Access (OEA) at or visit OEA.
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SUNY Research Foundation: A Great Place to Work.
The starting salary range (or hiring range) to be offered for this position is noted below, it represents SBU's good faith and reasonable estimate of the range of possible compensation at the time of posting.
