Scientist, Protein Science - Rockville, United States - Hansoh Bio

Hansoh Bio

Verified Company

Rockville, United States

1 day ago

Posted by:

Mark Lane

beBee recruiter

Description

HANSOH BIO

BIOTHERAPEUTICS - MARYLAND R&D CENTER

THE POSITION - SCIENTIST / SENIOR SCIENTIST

RESPONSIBILITIES

Establish and develop efficient molecular biology capabilities to generate DNA constructs for transient mammalian cell expression of antibodies and proteins to support therapeutic discovery and engineering efforts
Build consistent and robust protein expression capabilities for transient 293 and CHO cells that encompass HTP small scale through gram level protein production
Perform antibody characterization utilizing ELISA, Octet or Biacore
Perform _in vitro_ cellbased experiments using standard immunological, cellular and molecular biology techniques such as Western Blots, PCR/qPCR, ELISA, Luminex, flow cytometry, spectroscopy, and microscopy
Design key scientific experiments together with project teams
Desire and ability to learn new skills to help contribute to our multidisciplinary teams
Provide timely communication and presentations to project team and governance body
Assist with general lab management and upkeep including maintenance of biological samples, lab supplies, biological and chemical wastes, and calibration of instruments, in accordance with established guidelines
Diligent maintenance of lab records and logs in accordance with the standard practices
Other related research functions as deemed necessary to support daytoday functioning of the lab

QUALIFICATIONS

PhD. degree in chemical biology, molecular biology, cell biology or equivalent scientific discipline from an accredited college or university with mínimal 2 years of relevant experience
B.S. or M.S. degree in chemical biology, molecular biology, cell biology or equivalent scientific discipline from an accredited college or university with 10 years (with B.S.) or 5 years (with M.S.) of handson laboratory experience in an industrial or academic setting
Experience with various _in vitro_ and cellbased assay, mammalian cell culture, maintenance of cell lines, transfection, cellbased functional assays, flow cytometry, ELISA, Western Blots and PCR/qPCR
Experience with antibody characterization (LC-MS, HPSEC, Octet, Biacore) highly desired
Experience with _ÄKTA_ purification system or iQue3 preferred
Familiar with GraphPad Prism software
Experience in cloning of antibody into expression vector, display technology, antibody humanization and affinity optimization preferred
Proficient in computers with excellent record keeping, documentation and good organizational skills
Professional verbal and written communication and interpersonal skills, including ability to interpret and understand SOPs and laboratory procedures
Detailoriented, analytical with excellent trouble shooting skills
Flexible, forward thinking and motivated with ability to act independently

Pay:
$95, $115,000.00 per year

Experience:

- antibody characterization (LC-MS, HPSEC, Octet, Biacore): 2 years (required)
- cloning of antibody into expression vector: 2 years (required)
- antibody humanization and affinity optimization: 1 year (required)

Ability to Commute: