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    Research Technician II - Rochester, United States - University of Rochester

    University of Rochester
    University of Rochester Rochester, United States

    1 week ago

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    Description

    POSITION SUMMARY:


    The laboratory's research focuses on understanding the mechanisms and treatments of chronic obstructive pulmonary disease/emphysema, lung cancer, asthma, and pulmonary fibrosis.

    The position will also be responsible for carrying out the laboratory experiments _in vitro_ cell culture and _in vivo_ (cigarette smoke, flavoring chemicals, e-cigarette and waterpipe/hookah exposure to mice and procedures); assisting several technicians and postdoctoral fellows in the laboratory; and managing all aspects of animal protocols and laboratory work from data generation to data analysis
    . The execution of experiments will require proficiency in cell and molecular biology techniques.


    TYPICAL DUTIES:

    • Plans and conducts standardized technical procedures and lab experiments

    Techniques to be performed:

    Western blotting, immunostaining, lung function measurements experiments, ELISA, tissue section staining, Diff-Quick staining, immunohistochemistry, RNA isolation and DNA isolation, RT-PCR, and analyzing results and record keeping.

    Performs new experiments and assays in cells and tissues. Engages in experimental design and independent execution of above methods/protocols/assays.

    Assists and trains undergraduate students, graduate students and postdoctoral fellows to learn and perform assays, routine techniques/methods, and procedures for their experiments.

    Trains new laboratory personnel in fundamental lab techniques.

    Works with students and fellows/coordinators on basic and clinical research projects in collecting biological samples and performs experiments and assays (cell biology, biochemical and molecular biology techniques) in cells and biological tissues.


    • Maintains the colonies of transgenic and knockout mice; breeding of mice, record-keeping, animal protocols submissions/modifications, working closely with Vivarium and DLAM staffs, cigarette smoke inhalation exposure to mice and carrying out the laboratory experiments _in vitro_ and _in vivo_ (cigarette smoke and e-cigarette aerosol exposure to mice) exposing cigarette smoke to mice, preparing cigarette smoke extracts

    Animal Care:

    animal cigarette smoke exposures and inhalation (oral, s.c., i.m., i.v., or i.p.), lung and heart removal, dissection and tissue preparation.

    Maintains UCAR paperwork/protocols, orders new rodents, checks animals daily, oversight of DLAM for their maintenance of one animal room.

    Keeps track of various training and annual updates for all laboratory personnel so that the labs are in compliance with these offices.

    Ensures all other laboratory personnel are up-to-date on required laboratory and animal usage training. Keeps track of Institutional Biosafety Committee approvals, EH&S, and DLAM/UCAR training and annual updates for laboratory personnel and compliance. Coordinates clinical studies with clinical research center and the lab, assists in subjects recruitment and collection of biological samples.


    • Orders equipment and laboratory supplies, sets up experiments, cleans and performs routine maintenance on equipment. Places orders for animals and lab supplies, schedules maintenance for lab instruments, oversight and routine biochemical and molecular biological assays. Files order forms, keeps schedule for equipment maintenance, maintenance of computer records for lab and equipment, maintenance of chemical inventory and autoclave items, interacts with sales representatives, some cleaning and storing of glassware, oversight and record keeping for hazardous waste disposal. Plans and conducts standardized technical procedures and lab experiments. Sets up and operates equipment or instrumentation. Oversees and maintains laboratory. Serves as liaison of laboratory to Departmental and University administration.
    • Maintains laboratory supplies and inventories (chemicals, animals and equipment). Manages and maintains laboratory inventory and address needs for instrument repair.
    • Participate in lab meetings and seminars. Assist the Principal Investigator with preparing figures, data sets and written text for grants and publications.
    Other projects and job duties as assigned and as needs arise.


    QUALIFICATIONS:

    • High school diploma or GED required.
    • Associate's degree in Health Science, Biology, Chemistry, or other science related field of science preferred.
    • 1 year experience performing basic sampling, monitoring or research activities, compiling and collecting data and preparing reports; or equivalent combination of education and experience required.
    • Experience in animal (mouse) work and cell culture work with handling multi-tasks. Experience in routine biochemical and molecular biological techniques, and practical laboratory and animal experience preferred
    • Knowledge of OSHA safety requirements and scientific methods required required.
    • Strong interpersonal and commuication skills, including written communication required.
    • Must be willing to work with human biological samples, handling animals (mice) and animal tissues required.
    • Good organizational skills, ability to use good judgement, and demonstrate attention to detail are necessary required.
    • Experiential knowledge of the workings of an academic research laboratory, including general knowledge of biochemistry, microbiology, and molecular biology methods. Microscopy skills are beneficial preferred.
    • Prior experience in handling research animals, tissue culture, molecular and cell biology methodology and/or knowledge of immunologically-based techniques preferred.
    • Ability to work independently and the capacity to design, organize, and analyze experimental data that serves as an invaluable tool in the publication of papers in scientific journals and obtaining future funding required.
    • Prior experience working with human biological samples preferred.


    The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University's mission to Learn, Discover, Heal, Create - and Make the World Ever Better.

    In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law.

    This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations.


    How To Apply
    All applicants must apply online.

    _EOE Minorities/Females/Protected Veterans/Disabled_


    Pay Range

    Pay Range:
    $ $27.12 Hourly

    _The referenced pay range represents the minimum and maximum compensation for this job.

    Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.

    _

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    Location: School of Medicine & Dentistry


    Full/Part Time: Full-Time


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