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    Research Professional I - Athens, United States - University of Georgia

    University of Georgia
    University of Georgia Athens, United States

    3 weeks ago

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    Description
    Position Information

    Classification Title Research Paraprofessional/Professional FLSA Non-Exempt FTE 100 Minimum Qualifications

    Requires technical knowledge in the field acquired through an apprenticeship, a 2-year degree, or professional certificate. Please contact your Human Resources office for an evaluation of education/experience in lieu of the required minimum qualifications.

    Preferred Qualifications Position Summary


    Works independently and with others to design and develop components of on average five to eight ongoing projects, and provides supervision and guidance for graduate students, veterinary students, and other technicians within the department.


    Work involves: 1) characterization of vaccine candidates for Cytauxzoon felis including determination of intron/exon structure, sequence conservation across geographic isolates, expression of candidates within different parasite life-stages using novel techniques such as RNAScope in situ hybridization, and microscopic laser capture of tissue life-stages to assess vaccine candidate expression using quantitative real-time PCR (qRT-PCR), 2) optimization of PCR for antigen receptor rearrangement (PARR) from various tissue types as an aid in the diagnosis of lymphoma in cats and dogs, 3) design and optimization of experiments for analysis of potential mediators of progression of feline chronic kidney disease using qRT-PCR, 4) execution of canine clinical flow cytometry as an aid in the diagnosis of hematopoietic neoplasia, 5) optimization of feline clinical flow cytometry, 6) recombinant vaccine candidate production and purification for assessment in vitro using ELISPOT and Luminex assays, and 7) performance of flow cytometry for assessment of platelet function in dogs.


    Performance differs from that of a technician in the planning and executing complex experiments, analyzing data, and applying theoretical concepts for the science.

    Knowledge, Skills, Abilities and/or Competencies

    Molecular biology techniques - PCR, RT-qPCR, nucleic acid extraction, electrophoresis, cloning, protein isolation, and electrophoresis, immunoblotting. Cell culture - isolation and maintenance of cells, media preparation, and cryopreservation.

    Physical Demands

    Be able to sit or stand for extended periods of time

    Is driving a responsibility of this position? No Is this a Position of Trust? Yes Does this position have operation, access, or control of financial resources? Yes Does this position require a P-Card? No Is having a P-Card an essential function of this position? No Does this position have direct interaction or care of children under the age of 18 or direct patient care? No Does this position have Security Access (e.g., public safety, IT security, personnel records, patient records, or access to chemicals and medications) Yes Credit and P-Card policy

    Be advised a credit check will be required for all positions with financial responsibilities. For additional information about the credit check criteria, visit the UGA Credit Background Check website.

    Background Investigation Policy


    Offers of employment are contingent upon completion of a background investigation including, a criminal background check demonstrating your eligibility for employment with the University of Georgia; confirmation of the credentials and employment history reflected in your application materials (including reference checks) as they relate to the job-based requirements of the position applied for; and, if applicable, a satisfactory credit check.

    You may also be subject to a pre-employment drug test for positions with high-risk responsibilities, if applicable. Please visit the UGA Background Check website.

    Duties/Responsibilities

    Duties/Responsibilities


    Planning and executing data analysis and summary of molecular biology: DNA/RNA extraction, cloning-homologous recombination/TA cloning, agarose gel and capillary gel electrophoresis, PCR design and execution (primer design, reaction optimization) including standard PCR, qRT-PCR and PCR for antigen receptor rearrangement (PARR), sequence analysis, recombinant protein expression, immunoblotting, in-situ hybridization including RNAScope, Protein isolation and electrophoresis, characterization of vaccine candidates for Cytauxzoon felis including determination of intron/exon structure and sequence conservation across geographic isolates, assessment of expression of candidates within different parasite life-stages using novel techniques such as RNAScope in situ hybridization, optimization of PCR for antigen receptor rearrangement (PARR).

    Training of students and other staff for many of these experiments is expected. Reproducibility of data and clear presentation of data will be a measure of success.

    Percentage of time 50 Duties/Responsibilities


    Planning and executing data analysis and summary of cell biology techniques: Execution and optimization of veterinary clinical flow cytometry - Canine and feline clinical flow cytometry including a 12 antibody panel- isolation and labeling of cells, acquisition of data using an LSRII cytometer, optimization of feline clinical flow cytometry panel, media/reagent preparation.

    Isolation and purification of peripheral blood mononuclear cells for ELISPOT and Luminex assays. Reproducibility of data and clear presentation of data will be a measure of success. Training of students in these techniques is expected.

    Percentage of time 35 Duties/Responsibilities

    Laboratory management:

    Establishing and editing SOPs, providing guidance for proper storage/submission of clinical flow cytometry samples to the members of the CVM/VMC and outside veterinarians, autoclaving, glass washing, stock ordering, and inventory organization, preparation of solutions, and equipment maintenance.

    Provides training to graduate students and technicians in these areas.

    Percentage of time 15

    Contact Information

    Recruitment Contact

    Contact Details Recruitment Contact Name Recruitment Contact Email Recruitment Contact Phone

    Posting Specific Questions

    Required fields are indicated with an asterisk (*).

    Applicant Documents

    Required Documents


    • Resume/CV
    • Cover Letter
    Optional Documents

    Persons needing accommodations or assistance with the accessibility of materials related to this search are encouraged to contact University HR ).

    The University of Georgia is an Equal Opportunity/Affirmative Action employer.

    All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, ethnicity, age, genetic information, disability, gender identity, sexual orientation or protected veteran status.

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