Laboratory Technician - Research Triangle Park, United States - 22nd Century Technologies, Inc.

Description

*Duration: *3 Years to Permanent

*Overall Position Summary and Objectives

• The purpose of this position is to support research in our lab which includes coordination of research tasks with PI and other members of the lab, conducting experiments, generating, analyzing and interpreting data as well as drafting data and method summaries for scientific presentations and publications.
• This position will support development of advanced complex 2D co-cultures/3D in vitro models of the female reproductive tract using human cell cultures.
• The position will support experiments using DNA, RNA/transcriptomics, RT-PCR, protein/westerns and immunofluorescence/confocal approaches for molecular mechanistic and translational toxicology research.
• The position will provide technical mentoring to trainees and other lab staff.

*Min Education:

• Bachelor's

*Certifications & Licenses:

• Biosafety Level 1 (BSL 1)
• Biosafety Level 2 (BSL 2)

*Skills:

• Animal Handling: Mouse
• Equipment Maintenance
• Inventory Management
• Manuscript writing
• Ordering Supplies
• Data Presentation
• Data Analysis
• SOP writing
• Pipetting
• Polymerase chain reaction (PCR), qPCR, RT-PCR, RT-qPCR
• Gel Electrophoresis
• SDS-PAGE
• Nucleic Acid Extraction
• DNA Purification
• RNA purification
• Nucleic acid quantification
• Transfections
• Cell culture
• Primary cell culture
• 3D-Cell Culture
• Microarray
• Enzyme-Linked Immunosorbent Assay (ELISA)
• Western Blotting

*Software:

• Power point
• POTS
• Outlook
• Prism Graph pad
• Delta Graph
• Excel
• Word

*Field of Study:

• Biology
• Environmental Science
• Molecular Biology

*Statement of Work Details**Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform fundamental procedures required for research projects.
• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Follow established procedures and protocols; utilize aseptic technique to perform experiments.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform experiments on samples according to protocols.

*Operates laboratory machines and instruments; and prepares specimens and equipment for use.

• Work with staff on the maintenance of laboratory cell lines.
• Prepare materials and equipment for experiments.
• Maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining.
• Ensure that all specimens and equipment necessary for experiments are available and in good order.
• Perform processing, description and analysis of specimens.

*Analyzes, documents and reports on all procedures performed.

• Maintain and update laboratory notebooks and databases; and enter experimental data into spreadsheet and conduct statistical analysis.
• Collect data and prepare for analysis and interpretation.
• Maintains inventory of laboratory supplies.
• Cleans and sterilizes instruments, glassware and equipment.
• Clean and sterilize laboratory equipment such as glasses, metal instruments, sinks, tables and test panels.
• Ensure that adequate clean inventory is available for immediate usage.

*Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform fundamental procedures required for research projects.
• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Follow established procedures and protocols; utilize aseptic technique to perform experiments.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform flow cytometry-based experiments, including cell preparation, cell sorting and molecular analysis of sorted subsets.

*Maintains inventory of laboratory supplies and initiates purchase orders.

• Monitor and inventory routine laboratory supplies; ensure that supplies are stocked and organized.
• Manage the laboratory logistics and supply flow by tracking the consumption of laboratory materials.
• Inventory laboratory supplies and equipment, make recommendations and prepare purchase requests.
• Work with staff to maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining.
• Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results.

*Performs routine equipment maintenance.

• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Perform regularly scheduled maintenance on and generate maintenance reports for equipment.
• Carry out calibration; organize and initiate service and preventative maintenance on equipment.
• Monitor and maintain contracts for laboratory equipment and monitor equipment performance and maintenance schedule.

*Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

• Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
• Perform a variety of laboratory techniques and procedures.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform routine analysis and tests on specimens in the laboratory.
• Perform experiments on samples according to protocols.
• Performs advanced equipment maintenance.
• Monitor and maintain contracts for laboratory equipment and monitor equipment performance and maintenance schedule.
• Provides insights and expertise and recommends new strategies and approaches.
• Evaluate new types of experimental approaches/analyses and recommend changes to protocols.
• Determine when modifications and adjustments are necessary for experiments.
• Identify pitfalls in the original choice of methods or experimental direction and redirect the study to achieve improved results.
• Troubleshoot issues with technical procedures; utilize trial and error methods customize protocols to the biological systems utilized in the laboratory.

TS\_ST

*About our Company: -22nd Century Technologies Inc., (TSCTI) is one the fastest growing healthcare staffing companies in the United States. With presence in all 50 States and 6000+ employees nationwide, we have been providing unparallel healthcare and technology staffing services to Public and Private sectors for over 26 years.

Started as a technology staffing company in 1997, we have significantly expanded our healthcare practice to both Clinical and Non-Clinical staffing, serving various facilities, healthcare organizations and government agencies.

Our ISO certified staffing practices and technology driven staffing procedures – from recruitment to onboarding, along with an internal pre-vetted resume database of healthcare professionals, enable us to serve our customers with their immediate staffing needs.

TSCTI has extensive experience providing Physicians, Nurses, Pharmacists and other Allied Health professionals to various states – California, Connecticut, District of Columbia, Florida, Michigan, Minnesota, New Jersey, New York, Texas, Vermont, Virginia to name a few, and Our valued relationships with employees as well as commitment to meet and exceed clients' expectations through our exemplary employees proves our competency in today's healthcare staffing industry.

Recognized among *"Best Company to Work For" by Forbes, 22nd Century Technologies, Inc.,* consistently exceeds our clients' expectations by focusing on their absolute satisfaction with jobs while keeping our employees motivated.

*All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or protected veteran status.


Job Types:
Full-time, Contract


Pay:
$57, $62,000.00 per year


Schedule:

• 8 hour shift
• Day shift
• Monday to Friday

Ability to Relocate:

• Research Triangle Park, NC 27709: Relocate before starting work (Required)

Work Location:
In person