- Participate in the design, planning and execution of experiments.
- Make observations, analyze results, and maintain a detailed laboratory journal.
- Summarize results and create presentations for internal group meetings.
- Work with other members of the research team and within GCTx to accomplish company goals.
- Follow all safe laboratory practices and company policies.
- Execute experiments to support Cell Line Development and Scale up of PSC-derived cells.
- Perform experimental endpoint analysis on iPSC and differentiated derivatives including flow cytometry, RT/dd-PCR and microscopy.
- Support key lab processes, such as formulating culture media and preparing aliquots.
- Collaborate with PD and R&D teams to transfer procedures and protocols between teams.
- Assist PD scientists in execution of experiments.
- Effectively communicate results, in both written format and oral presentations.
- Follow concise protocols for reproducibility of cell culture processes.
- Maintain extensive records based on CMC guidelines.
- Perform DOE studies, when necessary, to optimize parameters affecting engineered cell line differentiation and stability.
- BSc with 3+ years or MSc with 1+ years of relevant industry experience in molecular and cellular biology, bioengineering, biochemistry, or related discipline.
- Experience with PSC cell culture, expansion, banking, and differentiation is a must.
- Hands-on experience in molecular biology laboratory techniques.
- Hands-on experience in analytical techniques relevant to the characterization of PSCs.
- Strong project management and organizational skills, with ability to prioritize and multitask.
- Effective communicator with excellent interpersonal skills.
- Ability to work in a laboratory environment approximately 75% of the time including weekend hours.
- Hands-on experience with multi-tray flasks is desirable.
- Experience with Single-Use technologies is desirable.
- Experience in gene expression analysis and next generation sequencing is desirable.
- Familiarity with techniques used to measure genome integrity and off target characterization is desirable.
- Familiarity with automation and cell cloning.
- Ability to conduct high quality presentations at internal meetings.
- Familiarity with working in cleanrooms under cGMP guidelines is desirable.
- Basic knowledge of cell culture manufacturing principles, risk assessments (FMEA), Chemistry, Manufacturing, and Controls (CMC) principles, and tech transfer.
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Research Associate/ Senior Research Associate - Cambridge, United States - GC Therapeutics
Description
Research Associate/Senior Research Associate, Process Development
Company Overview
GC Therapeutics is the first genome-wide cell landscape exploration company using an integrated synthetic biology and AI-driven platform for cell programming. Its patent-pending and proprietary pluripotent stem cell differentiation technology platform TFomeTM was developed in Professor George Church's lab, a pioneer insynthetic biology, and allows for the development of unique cell therapies with significantly streamlined manufacturing, improved cell quality, efficiency, and speed. GC Therapeutics is based in Cambridge, MA.For additional information, please visit
Job Purpose
We are seeking a talented and detail-oriented individual to join our growing stem cell research group as a Research Associate. This candidate will be responsible for the maintenance, differentiation, and functional analysis of pluripotent stem cell (PSC) lines and their derivatives to support the development of cell-based therapeutics. The candidate should be familiar with the concept of Design of Experiments (DOE).
Key Responsibilities
General:
Experimental Techniques:
Qualifications
Essential Qualifications:
Additional Qualifications:
Equal Opportunity Workplace: GC Therapeutics is an equal opportunity employer. We provide equal employment opportunities to all applicants for employment and existing employees without regard to ancestry, national origin, place of birth, race, color, gender, sexual orientation, marital status, pregnancy, religion, age, disability, gender identity, results of genetic testing, service in the military or otherwise to the full extent of all federal, state and local laws. GC Therapeutics' equal employment opportunity policy applies to all terms and conditions of recruiting, hiring, placement, training, compensation, transfer, leave of absence, employment, promotion, layoff and termination of employment.
