Sea On Lee

Ph.D. in Chemistry (specialized in synthetic biology) - Johns Hopkins University

Highly autonomous, efficient, and creative with years of experience in molecular biology and synthetic biology, focusing on biopolymers. 

Scientific mentorship experience in training 2 graduate students and 2 undergraduates. 

Collaborative research experiences with engineering labs for communal research goals. 

Extensive experience in designing, synthesizing, and characterizing target DNA, RNA, and protein products with synthetic, analytical, and theoretical skill sets. 

Detailed proficiency: cloning, plate reader assays, directed evolution, protein expression and purification, TEM imaging, enzyme activity assays, SDS-PAGE, RNA synthesis and extraction, Western blotting, northern blotting, UV-Vis, NMR, Mass-Spec (application), and IR. 

Experienced with analyzing tools: GraphPad Prism, SnapGene, BLAST, PyMOL, Image Lab 

Experienced with several programming languages: R, Mathematica, Python, GML 

Experienced with illustrative tools: Illustrator

• Job Experience (Scientific Developer)

Developing device control software for harvesting recombinant proteins from living bacterial and mammalian cells.

• Publication 

1. “Optimized Loopable Translation as a Platform for the Synthesis of Repetitive Proteins” – 2021 ACS Central Science. Sea On Lee, Qi Xie, Stephen Fried 
2. “A proteome-wide map of chaperone-assisted protein refolding in a cytosol-like milieu” – 2022 PNAS. Philip To, Yingzi Xia, Sea On Lee, Taylor Devlin, Karen G. Fleming, Stephen D. Fried 
3. “An Error Prone PCR Method for Small Amplicons” – 2021 Anal. Biochemistry. Sea On Lee, Stephen Fried 
4. “Secretion-Catalyzed Assembly of Protein Biomaterials on a Bacterial Membrane Surface” – 2023 JACS. Qi Xie, Sea On Lee, Nitya Vissamsetti, Stephen D. Fried 
5. “DNA increases the β-phase content of PVDF films” – 2020 CEIDP. Valerie Rennoll, Sea On Lee, Ugur Erturun, Stephen Fried, James West

• Patents 

Optimized mRNA Platform for Loopable Translation – JHU Reference C16770 (In conversion to a US Patent) Sea On Lee, Stephen Fried

• Johns Hopkins University Baltimore, MD Ph.D. Chemistry (specialized in synthetic biology) 2022 
• Virginia Polytechnic Institute and State University Blacksburg, VA Double B.S. Degrees in Biochemistry and Human Nutrition (GPA: 3.89/4.0) 2010-2014; Minor in Chemistry