Senior Scientist - Ridgefield
4 hours ago
Job description
Senior Scientist needed
Please do not apply if you have a PhD The hiring manager is only interested in candidates with a MS or BS at this time.
Duties:
Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq.
Documents and maintains detailed laboratory records in accordance with policies;
Operates and maintains lab equipment in collaboration with other lab members;
Assists acquisition of necessary supplies and equipment;
Defines and performs appropriate QC measures;
Follows relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field;
Demonstrates the ability to interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation;
Communicates own work effectively orally and in writing; contributes to writing protocols, procedures, and technical reports
Automate processing and results reporting and delivery;
Reports and treats data with a high level of integrity and ethics.
Skills:
CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins
Next-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)
Single cell technologies - Personal experience with sample prep, library generation, and quality control
Standard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresis
Cell culture - Proficient in maintaining multiple cell lines, transfections, and sterile technique
Experience in automation is a plus;
Able to perform variety of FGS experimental techniques at the same time;
Demonstrated experience working in a multi-disciplinary and collaborative environment;
Ability to troubleshoot FGS experiments both individually and as part of a team.
Excellent oral and written skills with the ability to communicate in an open, transparent, timely and consistent manner.
Education:
Master's degree from an accredited institution with 6+ years of experience in molecular, cell biology, or related scientific discipline.
Minimum of 2 years of Crispr experience.
Molecular biology technique experience including qPCR, western blot, and cell culture (primary cell lines).
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