Jobs
>
Cleveland

    Research Assistant 2 - Cleveland, United States - Case Western Reserve University

    Case Western Reserve University
    Case Western Reserve University Cleveland, United States

    3 weeks ago

    Default job background
    Description
    Job Description


    POSITION OBJECTIVE


    Working under general supervision, the Research Assistant 2 will plan and carry out basic and preclinical research that endeavors to further delineate virus and host mechanisms that could be exploited to purge persistent reservoirs of HIV in people with HIV (PWH) on lifelong antiretroviral therapy.

    The research assistant will perform viral reactivation experiments in an ex vivo primary cell model of HIV latency as well as using T cells isolated from PWH.

    These experiments will largely entail utilization of immunofluorescence flow cytometry, high resolution microscopic imaging and RT-qPCR assays.

    The research assistant will also support students in their assigned research projects by providing isolated primary cell samples and guidance through the utilization of conventional laboratory techniques.


    ESSENTIAL FUNCTIONS

    • Plan and carry out project in accordance with general plans such as performing experiments using molecular biology technologies, viral and bacterial cultivation and basic bioinformatics. (10%)
    • Assist in the routine preparation of latently infected primary CD4+ T cells carrying non-infectious HIV and further improvement of this procedure. May assist in developing improved techniques or procedures. (15%)
    • PBMC isolation from peripheral blood samples, CD4+ T cell isolation and culture. (10%)
    • Conduct a series of experiments to supply scientific information as part of a larger project, including performing highly reproducible RT-qPCR analysis of PWH samples with detailed record keeping. (15%)
    • Perform immunofluorescence flow cytometry and microscopy. (10%)
    • Collect and analyze data. Prepare figures following data collection and analysis while assisting in writing related to experiments performed. (10%)
    • Participate in lab meetings and read applicable literature. (10%)
    • Oversee inventory and maintain lab supplies (ordering, stocking, preparing solutions/media). Maintain general lab environment, including, but not limited to, equipment maintenance and scheduling repairs, organization and cleanliness of the lab. Maintain and ensure compliance with biosafety and laboratory regulations. (15%)
    NON
    ESSENTIAL FUNCTIONS

    • Train new staff; direct the work of students. Support students in their assigned research projects through training and provision of isolated primary cell samples. (5%)
    • Perform other duties as assigned such as liaising with Sales Representatives and Field Application Scientists to learn about new lab products and services. (<1%)

    CONTACTS

    Department:
    Daily contact with supervisor to discuss research and maintain workflow.

    University:
    Occasional contact with other departments to share information.

    External:
    Limited or no contact with vendors to exchange information.

    Students:
    Regular contact with student employees to exchange information.


    SUPERVISORY RESPONSIBILITY
    This position has no direct supervision of staff employees. Train new staff; direct the work of students.


    QUALIFICATIONS

    Education/Experience:

    Bachelor's degree in Biochemistry, Molecular Biology, Microbiology or a related field and 1 to 3 years of experience, or an Associate's degree in an approved biotechnology program and 2 to 4 years of experience required.

    Master's degree preferred.


    REQUIRED SKILLS

    • Has knowledge of commonly-used concepts, laboratory equipment, practices and procedures within the field of molecular biology and microbiology.
    • Experience in molecular biology techniques, excellent aseptic technique and general skills common to a microbiology lab are a must.
    • Strong documentation and organization skills with the ability to keep accurate records of all work performed.
    • Good analytical, statistical and presentation skills.
    • Proficient computer skills, particularly in the use of Adobe Illustrator, Photoshop and graphing software such as Origin or Prism.
    • Ability to meet consistent attendance in person.
    • Ability to interact with colleagues and the supervisor face to face.
    • Demonstrated willingness to learn new techniques with instruction.
    • Willingness to work in a biosafety level 2+ setting.
    • Relies on instruction and pre-established guidelines to perform the functions of the job,
    • Ability to operate laboratory equipment such as thermocylers, flow cytometer, UV/vis spectrophotometers, pH meter and autoclave.

    WORKING CONDITIONS
    General laboratory environment. May have exposure to chemical and biological hazards.

    Some of the work involving the handling and culture of cells obtained from virally suppressed PWH will be carried out in a biosafety level 2+ setting.

    Personal protective equipment, including lab coat, gloves and goggles, will be provided to limit exposure to hazards.

    Diversity Statement

    In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

    .

    Reasonable Accommodations

    Case Western Reserve University provides reasonable accommodations to applicants with disabilities.

    Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at to request a reasonable accommodation.

    Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.

  • Cleveland Clinic

    Research Assistant

    1 week ago

    Cleveland Clinic Cleveland, United States

    Join the Cleveland Clinic team, where you will work alongside passionate caregivers and provide patient-first healthcare. Cleveland Clinic is recognized as one of the top hospitals in the nation. At Cleveland Clinic, you will work alongside passionate and dedicated caregivers, re ...

  • Cleveland Clinic

    Research Assistant

    3 weeks ago

    Cleveland Clinic Cleveland, United States

    Join the Cleveland Clinic team, where you will work alongside passionate caregivers and provide patient-first healthcare. Cleveland Clinic is recognized as one of the top hospitals in the nation. At Cleveland Clinic, you will work alongside passionate and dedicated caregivers, re ...

  • Cleveland Clinic

    Research Assistant

    1 week ago

    Cleveland Clinic Cleveland, United States

    Join the Cleveland Clinic team, where you will work alongside passionate caregivers and provide patient-first healthcare. Cleveland Clinic is recognized as one of the top hospitals in the nation. At Cleveland Clinic, you will work alongside passionate and dedicated caregivers, re ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision the Research Assistant 2 will conduct projects within the aims of the laboratory's focus on the role of vitamin A and carotenoids in the functioning and maintenance of the retina and the adjacent retina pigm ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision, the Research Assistant 2 will plan and carry out projects in accordance with general plans. The lab performs advanced research at the intersection of cancer biology, intracellular signal transduction, and m ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision in the Department of Nutrition of the Case Western Reserve University School of Medicine, the Research Assistant 2 will plan and carry out research projects in mass spectrometry-based structural biology and ...

  • Case Western Reserve University

    Research Assistant 1

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Working under moderate supervision of the project manager, the Research Assistant 1 will perform research in accordance with IRB-approved research protocols to support a PCORI funded study focusing on fatigue management for people with multiple scler Research, Assistant, HR, Data ...

  • Case Western Reserve University

    Research Assistant 1

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under moderate supervision, the Research Assistant 1 will provide technical support and perform cancer-focused research with a selected faculty investigator to be determined. Working with the selected faculty member, the individual w ...

  • Case Western Reserve University

    Research Assistant 1

    5 days ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under moderate supervision, the Research Assistant 1 will perform research work in medical and related technical areas involving the use of lab/ research skills. The laboratory studies neural circuits in Drosophila using genetics, mo ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Working under general supervision, the Research Assistant 2 will plan and carry out cellular, molecular and animal research projects in accordance with general plans. The lab utilizes cellular and animal models for studying tumor progression, metasta Research, Assistant, Animal, ...

  • Case Western Reserve University

    Research Assistant 2

    2 days ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision, the research assistant will plan and carry out activities for a clinical research trial that involves exercise and psychotherapy in patients with comorbid pain and opioid addiction. Responsibilities include ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision, the primary role for this position is to coordinate a research study with the purpose of conducting a randomized control trial of a psychoeducational intervention designed to improve the self-efficacy of fa ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Working under general supervision, the Research Assistant 2 will perform and assist advanced research at the intersection of cancer biology, intracellular signal transduction, and membrane trafficking. The research assistant will join efforts to purs Research, Assistant, Public H ...

  • Case Western Reserve University

    Research Assistant 2

    5 days ago

    Case Western Reserve University Cleveland, United States

    Job Description · Position Objective · Working under general supervision, the Research Assistant 2 will plan and carry out research projects in accordance with general research guidelines. The responsibilities include planning and performing experiments on projects related to car ...

  • Case Western Reserve University

    Research Assistant 1

    5 days ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Under moderate supervision the Research Assistant 1 will perform experiments in a neuroscience lab researching synapse development and function. The position requires experience in a variety of approaches including routine molecular biology, ...

  • Case Western Reserve University

    Research Assistant 1

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under moderate supervision, the research assistant 1 will perform research work toward understanding the role of the GOLPH3 pathway in Golgi secretory trafficking and intracellular signaling. The research assistant will perform traff ...

  • Case Western Reserve University

    Research Assistant 1

    5 days ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under moderate supervision, perform research work in the biomedical field. The research lab develops nanotechnology tools to better understand cardiometabolic and neurological diseases such as atherosclerosis, type 2 diabetes, Alzhei ...

  • Case Western Reserve University

    Research Assistant 1

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under moderate supervision, the Research Assistant 1 will perform research work and support scientists by doing electrophysiological experiments using the marine mollusk (sea slug) Aplysia californica. The research assistant will ass ...

  • Case Western Reserve University

    Research Assistant 4

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working with a high degree of independence and under general direction, the Research Assistant 4 will coordinate major research activities to further develop novel therapeutic approaches to control inflammatory disorders while minimizing the ...

  • Case Western Reserve University

    Research Assistant 2

    3 weeks ago

    Case Western Reserve University Cleveland, United States

    Job Description · POSITION OBJECTIVE · Working under general supervision, perform research work in tissue preparation and biomedical imaging. The successful candidate will be part of a large research team with the goal of creating the world's first vagus nerve connectome. This wi ...