Research Associate, AAV Production - California, United States - Amber Bio

Description

Amber Bio is a biotechnology company pioneering new gene editing modalities using multi-kilobase edits to reach previously undruggable patient populations. Founded by pioneers in the CRISPR field from leading institutions for gene editing research, the company is developing a first-of-its-kind RNA writing platform that can correct thousands of bases at once, thereby correcting genetic mutations safely and reversibly. If you are interested in building a new frontier in genetic medicine, we welcome you to apply.

Job Description: AAV Production Research Associate I, II, III

Responsibilities:

• Support cell culture activities and perform cell culture experiments in multiple cell lines.
• Perform cell culture using Cellstacks for various cell types for AAV.
• Operate related equipment such as bioreactors, pumps, mixer, and AKTA.
• Knowledge of column AAV purification.
• Perform ddPCR, sequencing, and reporter-based assays and screening in mammalian cell lines, tissue samples.
• Conduct and troubleshoot experiments, independently and in collaboration with colleagues, to evaluate AAV vector candidates.
• Design and execute cell-based assays (AAV transduction, transfection, flow cytometry, luciferase assays, and immunofluorescence).
• Support the management of lab inventory and equipment, and assist with ordering lab supplies.
• Participate in vector design, cloning, transfection, viral transduction, cell culturing, and cell imaging.
• Demonstrated experience with standard molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence).
• Proactively troubleshoot technical issues and recommend potential corrective actions based on personal observations and literature searches.
• Prepare summaries of data and present internally to colleagues and management.
• Draft SOPs, follow protocols, diligently document experimental data in lab notebooks, be organized and maintain electronic work records.
• Author scientific reports and data summaries.
• Conduct assays and experiments using flow cytometry, PCR, cell-based assays/bioassay development, and ELISA techniques.
• Process tissues for RNA and DNA extraction.
• Collaborate with cross-functional teams to meet project goals, and advance novel vectors into pipeline.
• Strong written and verbal communication skills.

Qualifications:

• Bachelor's or Master's degree in Biology, Biochemistry, Chemical Engineering, Biological Engineering, or a related field.
• Required AAV production experience.
• Mammalian cell culture experience.
• Experience with standard molecular and cell biology techniques (plasmid design, cloning).
• At least 1 year of wet lab research experience.
• Critical thinker with excellent communication skills who thrives in a multidisciplinary, fast-paced team environment.

Preference will be given to those who display:

• High motivation, with a strong work ethic and dedication to generating impact.
• Attention to detail, with the ability to extract deep insights from data.
• Ability to go from ideation to data in an independent fashion.
• Long-term personal vision with defined career goals.
• High EQ with team-oriented thinking.
• Experience with CRISPR-Cas systems and/or gene editing and delivery technologies.
• Experience with lentivirus transduction.
• Experience with Flow Cytometry and data analysis.

If you have a passion for advancing gene editing technologies and desire to be part of a pioneering biotech company, we encourage you to apply and join our ambitious team.

Please apply directly through LinkedIn, or submit your resume and any additional relevant materials to

Amber Bio is an equal opportunity employer and encourages applications from candidates of diverse backgrounds. We value diversity and are committed to creating an inclusive and supportive work environment for all employees.