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- The established and primary mammalian cell cultures: ex vivo human AML expansion, other primary patient-derived cells cultures, clonal cells expansion, freezing cells, liquid nitrogen banking.
- Genetic manipulations with primary human cells and established cell lines (e.g., lentiviral transductions, transfections, CRISPR/Cas9, protein-coding gene expression, shRNA/siRNA gene silencing).
- Cell biology assays by immuno-staining (ICC, WB, Flow cytometry) and other techniques: cell viability (life/dead), apoptosis, proliferation.
- Assessment of protein and RNA expression in primary human, mouse tissues and established cell lines.
- Mice colonies maintenance, IV and other types of injections, analysis of the bone marrow, spleen, lungs etc, by flow cytometry and other assays.
- IRB, IACUC and other protocol's writing, laboratory management, and student's supervision.
- Ensure protocol adherence; review progress and evaluate results.
- Perform a variety of experimental tasks including preparing specimens, microscopy, surgical procedures.
- Execute and develop data collection procedures; record, correlate, and analyze data; prepare analysis summaries.
- Develop project protocols; provide input in determining feasibility of goals and objectives; responsible for assuring quality, cost effectiveness and timeliness of assigned projects.
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Research Technologist - University Park, United States - Penn State University
Description
APPLICATION INSTRUCTIONS:
JOB DESCRIPTION AND POSITION REQUIREMENTS:
The Penn State College of Medicine, Department of Pediatrics, in Hershey, PA is seeking a Research Technologist.
We are looking for an experienced researcher for pre-clinical AML studies, including mouse AML models, molecular biology, cell biology, and cancer biology assays.
The Research Technologist- Medical Science develops, designs and conducts clinical research projects/experiments; investigates and evaluates research methods, advises faculty/researchers on optimal use of methodology; collects, correlates, analyzes, summarizes and reports on results; identifies data integrity issues; trains users in equipment operation and research techniques; and oversees daily operations of projects.
Responsibilities include:
Bachelor's Degree
Minimum Experience
3+ years of relevant experience
Equivalency
Or an equivalent combination of education and experience
This is a limited-term position funded for one year from date of hire, with possibility of refunding.
The following clearances must be successfully completed for this position:
The Pennsylvania State University is committed to and accountable for advancing diversity, equity, and inclusion in all of its forms.
We embrace individual uniqueness, foster a culture of inclusive excellence that supports both broad and specific diversity initiatives, leverage the educational and institutional benefits of diversity, and engage all individuals to help them thrive.
We value inclusive excellence as a core strength and an essential element of our public service mission.The pay range for this position, including all possible grades is:
$42, $61,000.00
Salary Structure - additional information on Penn State's job and salary structure.
CAMPUS SECURITY CRIME STATISTICS:
Pursuant to the Jeanne Clery Disclosure of Campus Security Policy and Campus Crime Statistics Act and the Pennsylvania Act of 1988, Penn State publishes a combined Annual Security and Annual Fire Safety Report (ASR).
The ASR includes crime statistics and institutional policies concerning campus security, such as those concerning alcohol and drug use, crime prevention, the reporting of crimes, sexual assault, and other matters.
The ASR is available for review here.Employment with the University will require successful completion of background check(s) in accordance with University policies.
EEO IS THE LAW
Penn State is an equal opportunity, affirmative action employer, and is committed to providing employment opportunities to all qualified applicants without regard to race, color, religion, age, sex, sexual orientation, gender identity, national origin, disability or protected veteran status.
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