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Associate Researcher - San Diego - Net2Source (N2S)
Description
Details:
Job Title: Research Associate
Location: San Diego, CA 92131
100% Onsite
Duration: 9+ months contract
Pay Rate: $25 - $30/hr on w2
Description
Position Summary
The Structural Biology and Protein Sciences group is seeking a highly motivated contractor to support recombinant protein production efforts, primarily utilizing insect cell expression systems. The successful candidate will be responsible for maintaining eukaryotic cell cultures, generating and managing high-titer baculovirus stocks, and performing multi-liter scale protein expression and harvest workflows. Additional responsibilities include executing plate-based expression and purification profiling to enable rapid screening of construct and condition variants. This role requires strong organizational skills, attention to detail, and the ability to operate efficiently in a fast-paced research environment.
Key Responsibilities
• Maintain healthy eukaryotic cell cultures, including insect (Sf9, Sf21, High Five) and/or mammalian expression lines.
• Generate, amplify, and quality-control baculovirus stocks, including centralized storage and record management.
• Execute multi-liter scale recombinant protein expression experiments, including infection, culture monitoring, and cell harvest.
• Perform small-scale, plate-based expression and purification profiling to assess expression levels, solubility, and purification behavior.
• Maintain accurate and timely records of experimental data, cell line usage, and virus stock inventories.
• Operate and maintain basic laboratory instrumentation and contribute to general lab support activities as needed.
• Collaborate closely with team members to optimize expression workflows and troubleshoot technical issues.
Minimum Qualifications
• BS degree in Molecular Biology, Cell Biology, Biochemistry, or a related scientific discipline.
• Minimum of 3 years of hands-on laboratory experience, including work with insect cell culture (e.g., Sf9, Sf21, Hi5) and/or mammalian cell culture systems.
• Demonstrated experience in aseptic technique and routine maintenance of suspension cultures.
Highly Desirable Qualifications
• Experience with the Bac-to-Bac baculovirus expression system, including donor plasmid preparation, bacmid generation, and virus amplification.
• Familiarity with plate-based expression and purification approaches (e.g., 24–96 well plates and rapid IMAC and other affinity-based screens).
• Experience conducting multi-liter expression campaigns and associated downstream sample handling.
• Strong analytical, organizational, and documentation skills.
• Ability to work independently and in cross-functional teams.
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Associate Researcher
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Research Associate
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Research Associate
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Research Associate
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Research Associate
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Research Associate
Only for registered members San Diego
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Research Associate
Only for registered members San Diego
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Associate Researcher
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Research Associate
Only for registered members San Diego, CA
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Research Associate
Only for registered members San Diego