Associate Researcher - San Diego - Net2Source (N2S)

    Net2Source (N2S)
    Net2Source (N2S) San Diego

    1 day ago

    $55,000 - $90,000 (USD) per year * Pharmaceutical / Bio-tech
    Description

    Details:

    Job Title: Research Associate

    Location: San Diego, CA 92131

    100% Onsite

    Duration: 9+ months contract

    Pay Rate: $25 - $30/hr on w2

    Description

    Position Summary

    The Structural Biology and Protein Sciences group is seeking a highly motivated contractor to support recombinant protein production efforts, primarily utilizing insect cell expression systems. The successful candidate will be responsible for maintaining eukaryotic cell cultures, generating and managing high-titer baculovirus stocks, and performing multi-liter scale protein expression and harvest workflows. Additional responsibilities include executing plate-based expression and purification profiling to enable rapid screening of construct and condition variants. This role requires strong organizational skills, attention to detail, and the ability to operate efficiently in a fast-paced research environment.

    Key Responsibilities


    • Maintain healthy eukaryotic cell cultures, including insect (Sf9, Sf21, High Five) and/or mammalian expression lines.


    • Generate, amplify, and quality-control baculovirus stocks, including centralized storage and record management.


    • Execute multi-liter scale recombinant protein expression experiments, including infection, culture monitoring, and cell harvest.


    • Perform small-scale, plate-based expression and purification profiling to assess expression levels, solubility, and purification behavior.


    • Maintain accurate and timely records of experimental data, cell line usage, and virus stock inventories.


    • Operate and maintain basic laboratory instrumentation and contribute to general lab support activities as needed.


    • Collaborate closely with team members to optimize expression workflows and troubleshoot technical issues.

    Minimum Qualifications


    • BS degree in Molecular Biology, Cell Biology, Biochemistry, or a related scientific discipline.


    • Minimum of 3 years of hands-on laboratory experience, including work with insect cell culture (e.g., Sf9, Sf21, Hi5) and/or mammalian cell culture systems.


    • Demonstrated experience in aseptic technique and routine maintenance of suspension cultures.

    Highly Desirable Qualifications


    • Experience with the Bac-to-Bac baculovirus expression system, including donor plasmid preparation, bacmid generation, and virus amplification.


    • Familiarity with plate-based expression and purification approaches (e.g., 24–96 well plates and rapid IMAC and other affinity-based screens).


    • Experience conducting multi-liter expression campaigns and associated downstream sample handling.


    • Strong analytical, organizational, and documentation skills.


    • Ability to work independently and in cross-functional teams.

    * This salary range is an estimation made by beBee
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