Cell Biologist - Boston, Massachusetts, United States
2 days ago
Job description
Cell Biologist needs 3+ years for MS - 5+ years for BS. Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques.
Cell Biologist requires:
- Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting.
- Hands-on experience with cell delivery methods of gene editing tools.
- Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment.
- Strong organizational skills and diligent record keeping.
- Experience in pancreatic islet directed differentiation is highly desirable
- Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable.
- Mandatory weekend cell culture responsibilities on rotation basis.
Cell Biologist duties:
- Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells.
- Routine iPSC cell culture, passaging and media making.
- Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening and delivery.
- Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows.
- Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR.
- Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments.
- Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance.
- Collaborate cross-functionally to support transfer of R&D assays to GMP.
- Maintain accurate and detailed records of all experiments, synthesize results, prepare reports and present in meetings.
- Contribute to SOP development.
- Work seamlessly in executing workplans and meeting deadlines.
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