- DNA plasmid design & cloning
- Automated high throughput DNA purification
- High throughput molecular biology operations, including PCR, ligation reactions (Gibson & ITA), & DNA purification using liquid handling automation
- Sample preparation for sequencing
- DNA & RNA quantitation & QC
- Supporting transient mammalian transfections with DNA
- Organization and storage of DNA fragments/plasmids, including tracking using Genedata Biologics Software, as well as record keeping using electronic laboratory notebooks
- Close interaction with project teams
- Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods
Education & Qualifications - Bachelor's degree (0-2 years' experience) or Associate's degree (1-3 years' experience) with experience in Molecular Biology, Biochemistry, Biology, Biological Engineering or a related discipline along with a basic understanding of molecular biology and protein expression.
- PCR or Molecular Biology Experience
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Scientist - North Cambridge, United States - Randstad Life Sciences US
Description
TitleScientist
Location
Cambridge, MA
Pay
$30-33/hr
Contract
18+ months
Job Description
BioMedicine Design (BMD) High-Throughput Protein Production (HTP) Group is seeking a highly motivated scientist to support the molecular biology & protein production workflows: DNA construct design/assembly, PCR, sample preparation for sequencing, DNA production & quantitation, mammalian cell culture & transfections, protein purification & quantitation, and automated liquid handling. The candidate will play a major role in a core group that consistently delivers hundreds of protein samples per week to research teams across the department. The candidate will be responsible for generating DNA constructs and performing high-throughput processes for macromolecule production (DNA, RNA, and protein). The candidate will also be responsible for updating the laboratory database and relaying data in organized presentations to project teams.
Job Responsibilities
