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    Research Associate III - Bothell, United States - Actalent

    Actalent
    Actalent Bothell, United States

    4 weeks ago

    Default job background
    Full time Education / Training
    Description

    Description:

    An oncology biotech company is seeking a collaborative and independent Research Associate III (RA III) to participate in development of antibody-based oncology therapeutics. The RA III will report to the Molecular and Cellular Technologies team lead within Antibody Discovery. Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns. The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.

    Responsibilities:


    • Design, execute, troubleshoot, optimize, and interpret experiments


    • Order constructs for overexpression and knockout cell line generation


    • Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies


    • Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization


    • Perform routine cell culture maintenance and banking, working with both primary and cancer cells


    • Optimize and perform flow cytometry assays to screen and characterize antibodies


    • Maintain laboratory spaces, equipment, and supplies


    • Keep an up-to-date and accurate electronic laboratory notebook


    • Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings


    • Proactively seek guidance and build knowledge relevant to the role

    Additional Skills & Qualifications:


    • Bachelor's degree + 4 years laboratory research experience or Master's degree + 2 years laboratory research experience in biological sciences or related subject


    • Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions


    • Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR


    • Experience in multi-color flow cytometry


    • Demonstrated ability to work independently and collaboratively, according to project needs


    • Strong written and verbal communication skills, including ability to maintain a good working laboratory notebook


    • Experience with FACS and single-cell cloning


    • Basic molecular biology skills, including vector design, cloning, and PCR


    • Knowledge of antibodies or antibody drug conjugates (ADCs)

    About Actalent

    Actalent is a global leader in engineering and sciences services and talent solutions. We help visionary companies advance their engineering and science initiatives through access to specialized experts who drive scale, innovation and speed to market. With a network of almost 30,000 consultants and more than 4,500 clients across the U.S., Canada, Asia and Europe, Actalent serves many of the Fortune 500.

    Diversity, Equity & Inclusion

    At Actalent, diversity and inclusion are a bridge towards the equity and success of our people. DE&I are embedded into our culture through:

    • Hiring diverse talent
    • Maintaining an inclusive environment through persistent self-reflection
    • Building a culture of care, engagement, and recognition with clear outcomes
    • Ensuring growth opportunities for our people

    The company is an equal opportunity employer and will consider all applications without regard to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.

    If you would like to request a reasonable accommodation, such as the modification or adjustment of the job application process or interviewing process due to a disability, please email for other accommodation options.

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