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- Assumes shared responsibility for general laboratory maintenance by
- Maintains an excellent working knowledge of all basic laboratory equipment and apparatus.
- Maintains Lab Functions to include ordering of supplies and ensures lab equipment is maintained in good operating order.
- Requisition ID: 166361
- Employment Status: Full-Time
- Employee Status: Regular
- Work Week: Days
- Minimum Salary: US Dollar (USD) 43,500
- Midpoint Salary: US Dollar (USD) 54,500
- Maximum Salary : US Dollar (USD) 65,500
- Fund Type: Soft
- Work Location: Onsite
- Pivotal Position: Yes
- Referral Bonus Available?: Yes
- Relocation Assistance Available?: Yes
- Science Jobs: No
Research Assistant II - Texas, United States - University of Texas M.D. Anderson
Description
JOB SPECIFIC COMPETENCIES
Laboratory Technical Function
Experience with blood processing and library preparation pipelines.
i. Liquid biopsy
a. Isolates plasma and PBMCs from whole blood.
b. Isolates and stores extracellular vesicles and plasma DNA.
c. Annotates and stores of isolated analytes.
d. Performs RNA isolation from plasma and depletion of globin and ribosomal
RNA.
e. Performs library preparation from plasma derived RNA and DNA.
f. FFPE and FF tissue processing and storage.
ii. Mouse Models of pancreatic cancer
a. Performing and assisting in animal experiments (breeding, weening, ear tagging,
genotyping, Imaging, tissue harvesting, injections and record keeping of animal
inventory).
iii. Tissue processing
a. Receives, processes and store human tissue specimens
b. Performs tissue dissociation
c. Performs tissue sectioning and H&E staining
d. Assists graduate students and postdoctoral fellows in various molecular biology
assays such as; multiplex immunofluorescence, DNA, RNA and protein isolation
and western blots
e. Maintains pancreatic cancer cell lines using sterile technique. Extract protein
lysates, DNA and RNA from cells.
f. Performs library preparation for spatial transcriptomics using the 10X genomics
platforms.
g. Performs multiplex immunofluorescence assays such as COMET, OPAL and
IMC.
Laboratory Maintenance
b) performing record keeping to promote accountability and integrity in research,
c) monitoring work flow as it pertains to the need for supplies, equipment maintenance and preparation of stock solutions,
d) keeping ample stock of supplies on hand and performs periodic inventory to check stock supplies,
e) following up on orders to ensure organization and accessibility of all reagents.
Bachelor's degree with major course works in one of the basic sciences. One year experience in scientific or experimental research work. No experience required with preferred degree.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law.
Additional Information