Scientist or Senior Scientist - Cambridge, United States - HydroGene Therapeutics

Description

Company Overview:

HydroGene Therapeutics is a biotech startup specializing in cutting-edge gene therapy solutions for monogenic liver disorders. Our dedicated team of scientists is committed to advancing medical knowledge and transforming patient outcomes through innovative molecular interventions.

The Role:

We are seeking a highly skilled and motivated Scientist with extensive hands-on experience in molecular biology techniques. This individual will join the research team and play a key role in advancing our gene therapy research initiatives, contributing to the development of novel treatments for monogenic liver diseases. This is a full-time role that will be 100% on-site and based in Cambridge, MA.

Responsibilities:

Molecular Biology:

• Conduct DNA cloning and plasmid DNA preparation
• Perform gel electrophoresis, Western blot, and DNA/RNA extraction from cells and tissues
• Conduct polymerase chain reaction (PCR, quantitative PCR (qPCR) and RT-PCR.
• Execute genome editing with CRISPR technology.

Cell Biology:

• Cell culture: Cell line maintenance, transfection and electroporation
• In vitro cell based assays, experience with flow cytometry preferred

Histology:

• Sample preparations, immunohistochemistry, and immunofluorescence
• Conduct RNA in situ hybridization, RNA-scope preferred

Project Management:

• Execute benchwork independently, demonstrating a high level of technical proficiency
• Organize all research data, maintain electronic lab notebook
• Manage research projects from conception to completion

Team Collaboration:

• Train and supervise laboratory technicians, fostering a collaborative and efficient work environment

Qualifications:

• Ph.D. in a biomedical field with a focus on molecular biology with up to 3 years of research experience in the pharmaceutical/biotech industry or post-doctoral experience in relevant field. Candidates with 5+ years research experience in the pharmaceutical/biotech industry with a bachelor's degree or a master's degree with 3+ years research experience within the pharmaceutical/biotech industry will also be considered.
• Proven expertise in molecular biology techniques, including DNA cloning, DNA/RNA extraction, qPCR, western blotting
• Strong background in histology, immunohistochemistry, and immunofluorescence
• Ability to work independently, manage projects, and troubleshoot technical challenges
• Excellent communication and interpersonal skills
• Previous experience with mouse models is preferred

HydroGene Therapeutics is an equal opportunity employer. We celebrate diversity and are committed to creating an inclusive environment for all employees.