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    Development Of Vaccines Against HERV-K Envelope Technician - Bethesda, United States - Axle

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    Job Description

    Job Description

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    Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).


    Axle is seeking a Development Of Vaccines Against HERV- K Envelope Technician to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Neurological Disorders and Stroke (NINDS) located in Bethesda, MD.


    Benefits We Offer:

    • 100% Medical, Dental & Vision Coverage for Employees
    • Paid Time Off and Paid Holidays
    • 401K match up to 5%
    • Educational Benefits for Career Growth
    • Employee Referral Bonus
    • Flexible Spending Accounts:
      • Healthcare (FSA)
      • Parking Reimbursement Account (PRK)
      • Dependent Care Assistant Program (DCAP)
      • Transportation Reimbursement Account (TRN)

    Overall Position Summary and Objectives

    ​​The technician will support project development of vaccines against HERV-K envelope to treat amyotrophic lateral sclerosis. Transgenic mice expressing HERV-K envelope under a neuronal promoter will be immunized with the vaccine compounds before the appearance of clinical symptoms, to test the prevention capability of the vaccines.

    Deliverables:

    • ​Perform subcutaneous mice injections, collection blood, serum, plasma samples shipping - Daily
    • Meet with lab members to present updates - Weekly

    Work Details:

    • Track and maintain database for all research activities; update and maintain laboratory notebooks and databases; and record procedures performed in laboratory notebook. 1
    • Collect blood and tissue samples from animals. 2
    • Prepare animals for experiments. 3
    • Ensure the humane treatment of animals in the laboratory. 4
    • Provide technical guidance and assistance for researchers interested in well-established protocols. 5
    • Provides laboratory experience needed to maintain experimental animal colonies and collect sequential colony census.
    • Conduct small animal husbandry, animal handling, and specific procedures.
    • Interpret genotyping results and maintain genotyping records.
    • Perform genotyping PCR to maintain transgenic mouse colonies used in projects, including images of genotyping PCR gels and tabulation of genotyping results.
    • Perform animal handling and specific procedures.
    • Perform laboratory task in support of neurological studies in small animals.
    • Manage multiple animal studies running concurrently in different phases.
    • Perform laboratory and animal technical duties to include, but not limited to: weighing animals and feed, blood withdrawals, performing injections, operating behavioral equipment.
    • Maintain animal colonies and organization of animal inventory; monitor procedures; maintain the amounts of animals used and animal procurement.
    • Harvest and collect animal embryos, tissues, and organs.
    • Prepares / monitors caging environments and animal health.
    • Report any animal health, care, and/or well-being concerns to the appropriate facility management staff and facility veterinarians.
    • Collaborates with veterinarians in the performance of animal surgeries.
    • Prepare animals for surgery and ensure proper disposal of animal carcasses.
    • Handle, dissect and euthanize animals.
    • Maintains detailed and accurate records.
    • Collect both in vivo and ex vivo data; collect and maintain data using various systems.
    • Prepare reports for collected data which include representative data, conditions of data collection.
    • Maintain integrity of the research process by following good laboratory practices including: detailed record keeping of methods, routine animal observations, data collections.
    • Assists with organizing the shipment of mice to and from facility.
    • Work with staff on organizing the animal shipment to and from the facility.
    • Order and receive small animal shipments.
    • Maintains records of animal protocols.
    • Perform and maintain records of molecular biology protocols.
    • Maintains laboratory stock of reagents and materials.
    • Perform routine laboratory maintenance including laboratory stock of reagents and materials.
    • Check current inventory in the laboratory and order appropriate animals, media, buffers, reagents, tissue samples and other required materials.
    • Performs experimental procedures as well as standardized and specialized tests, exams and vaccinations.
    • Conduct in vivo drug studies in animal models.
    • Trains other in proper animal handing and surgical procedures.

    1, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those ranked



    Minimum Education

    High school


    Additional Qualifications:

    Certifications & Licenses
    • Animal work
    • Work with transgenic mice
    • Biological samples shipping

    Field of Study
    • Biology

    Software
    • Microsoft
    • Power point
    • Excel

    Skills
    • Mice injection
    • Injection vaccine to the mice
    • Mice handling
    • Collection mice blood, serum, plasma

    Disclaimer:The above description is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.


    The diversity of Axle's employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment based on age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.


    Accessibility: If you need an accommodation as part of the employment process please contact:

    #IND



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